In Vitro Mechanistic and in Vivo anti-tumor Studiesof Glycosmis Pentaphylla (Retz.)DC against breast cancer

Shoja, MH and Reddy, Neetinkumar D and Nayak, Pawan G and Biswas, Subhankar and Rao, Mallikarjuna C (2016) In Vitro Mechanistic and in Vivo anti-tumor Studiesof Glycosmis Pentaphylla (Retz.)DC against breast cancer. Journal of Ethnopharmacology, 186. pp. 159-168.

[img] PDF
626 DisplayPdf.pdf - Published Version
Restricted to Registered users only

Download (2MB) | Request a copy

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: Glycosmis pentaphylla (Retz.) DC (Rutaceae) has been traditionally used for the treatment of rheumatism, cancer, liver disorders, inflammation etc. AIM OF THE STUDY: The present study is aimed at elucidating the effect of Glycosmis pentaphylla (Retz.) DC on the key markers of apoptosis, metastasis and angiogenesis, in vitro. The study also evaluated the effect of fractions in vivo in DMBA-induced mammary tumor model.MATERIALS AND METHODS: Fractions of Glycosmis pentaphylla (Retz.) DC leaf extracts was studied for their effect on apoptotic markers in breast cancer cell lines, MCF-7 and MDA-MB-231 cells. They were also studied for their effect on metastatic and angiogenic markers, MMP-9 and HIF-1α in MCF-7 cells. The fractions were studied in vivo in DMBA-induced mammary tumor model in Sprague Dawley rats.RESULTS:The studies showed that the fractions induced apoptosis in breast cancer cells through the intrinsic/mitochondrial apoptotic pathway. The fractions were also able to inhibit the metastatic and angiogenic markers, MMP-9 and HIF-1α. Anti-tumor studies in DMBA-induced mammary model in Sprague Dawley rats also showed favorable results.

Item Type: Article
Uncontrolled Keywords: Glycosmis pentaphylla (Retz.)DC; Breast cancer; Apoptosis; Westernblot; Metastasis; Angiogenesis
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 08 Jun 2016 15:13
Last Modified: 08 Jun 2016 15:13
URI: http://eprints.manipal.edu/id/eprint/146324

Actions (login required)

View Item View Item