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Aqueous two phase systems for the recovery of biomolecules from tannery wastewater

Raja, Selvaraj (2013) Aqueous two phase systems for the recovery of biomolecules from tannery wastewater. Phd. Thesis thesis, Manipal Institute of Technology, Manipal.

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Abstract

The thesis deals with the development and evaluation of eco-friendly aqueous two phase systems composed of poly-ethylene-glycol (PEG) + sodium citrate (SC) + water to recover soluble proteins from a model tannery wastewater. Liquid- liquid equilibrium data of aqueous two-phase systems (ATPS) formed by sodium citrate (SC) and poly-ethylene-glycol (PEG) of different molecular weights (600, 1000, 2000, 4000, and 6000) with water were developed at different pH (6, 7, and 8) and constant temperature 25°C. Binodal data was described using a third order polynomial equation. An increase in molecular weight of PEG shifted the binodal curve to lower PEG and salt concentrations. An increase in pH showed similar shape and tend to merge at high concentration of PEG and salt. Tie-Line Length (TLL) and the slope of the tie-line (STL) were related to the equilibrium phase composition. The tie-lines were correlated using the Othmer-Tobias and Bancroft equations and the coefficient values were calculated. ATPS made up of PEG 2000, 4000 and 6000 + SC was used to recover the valuable biomolecules from a model tannery wastewater system. The effect of various protein partitioning parameters like PEG molecular weight (MW), concentration of phase forming components, system pH, temperature and concentration of sodium chloride were studied. In all the systems, the crude proteins preferentially partitioned towards the salt rich bottom phase. Furthermore, the thermodynamic parameters as a function of temperature were calculated for the PEG 6000/ SC system by using Van’t Hoff relationship. It was possible to recover 83.47 % in the bottom phase by employing an ATPS consisted of PEG MW 6000 20% (w/w) / SC 15% (w/w) / 0.3M NaCl at a temperature of 30°C and pH 8.

Item Type: Thesis (Phd. Thesis)
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 28 Jun 2016 14:49
Last Modified: 12 Jan 2017 14:51
URI: http://eprints.manipal.edu/id/eprint/146419

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