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Comparison of etest and agar dilution for determining minimum inhibitory concentration of vancomycin to healthcare

*, Jyoti Kumari and Shenoy, Shalini and *, Chakrapani M and *, Vidyalakshmi K and Bhat, Gopalkrishna K (2016) Comparison of etest and agar dilution for determining minimum inhibitory concentration of vancomycin to healthcare. Asian Journal of Pharmaceutical and Clinical Research, 9 (4). pp. 149-151. ISSN 0974-2441

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Abstract

Objectives: To compare agar dilution method and Etest in the determination of minimum inhibitory concentration (MIC) of vancomycin to healthcareassociated methicillin-resistant Staphylococcus aureus (HA-MRSA). Methods: A total of 98 non-duplicate strains of HA-MRSA isolated from different clinical specimens were tested for their antibiotic susceptibility pattern by Kirby-Bauer disk diffusion method and vancomycin MIC by agar dilution method and Etest (BioMerieux, France). Results: Out of 98 strains of HA-MRSA, 94 (95.9%) were vancomycin susceptible (MIC ≤2 μg/ml and 4 (4.1%) were vancomycin intermediate (MIC 4 μg/ml) by agar dilution method. By Etest, 53 (54.1%) were vancomycin susceptible, 4 (4.1%) were vancomycin intermediate, and the remaining 41 isolates had vancomycin MIC between 2 μg/ml and 4 μg/ml. Conclusion: Etest allows the detection of HA-MRSA strains with intermediate MIC values in addition to traditional dilutions. These properties will help in detection of MIC creep and also decision-making in using vancomycin for the treatment of serious infections caused by HA-MRSA.

Item Type: Article
Uncontrolled Keywords: Vancomycin, Minimum inhibitory concentration, Etest, Agar dilution.
Subjects: Medicine > KMC Mangalore > Microbiology
Medicine > KMC Mangalore > Medicine
Depositing User: KMCMLR User
Date Deposited: 29 Jun 2016 11:08
Last Modified: 29 Jun 2016 11:08
URI: http://eprints.manipal.edu/id/eprint/146440

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