Simultaneous estimation of emitricitabine, efavirenz, tenofovir disproxil fumarate by chemometry and reverse phase-high performance liquid chromatography

Kumari, Ramya G and Deepika, NC and Bhat, Krishnamurthy (2016) Simultaneous estimation of emitricitabine, efavirenz, tenofovir disproxil fumarate by chemometry and reverse phase-high performance liquid chromatography. Indian Drugs, 53 (6). pp. 62-69. ISSN 0019-462X

[img] PDF
RMS-0891.pdf - Published Version
Restricted to Registered users only

Download (429kB) | Request a copy

Abstract

A chemometric ultra-violet spectrophotometric method of analysis, Partial Least Square (PLS) method was applied to simultaneous assay of emitricitrabine, efavirenz and tenofovir disoproxil fumarate (DF) in their combined dosage tablet formulation. For comparison of this chemometric method a HPLC method for simultaneous determination of emitricitabine, efavirenz and tenofovir DF in combination was developed. Twenty one mixed solutions were prepared for chemometric calibration set and twenty one ternary mixtures were prepared as validation sets. The absorbance data matrix for training set was obtained by recording absorbance within wavelength range 230-290 nm at 2nm intervals. The developed multivariate and HPLC methods were successfully tested for laboratory mixtures as well as commercial tablet formulation of emitricitrabine, efavirenz, tenofovir DF.

Item Type: Article
Uncontrolled Keywords: Emitricitabine; efavirenz; tenofovir disproxil fumarate.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Library
Date Deposited: 20 Jul 2016 15:14
Last Modified: 20 Jul 2016 15:14
URI: http://eprints.manipal.edu/id/eprint/146672

Actions (login required)

View Item View Item