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Process optimization for tannas eproduction by Bacillus gottheilii M2S2 on inert polyurethane foam support

Selvaraj, Subbalaxmi and Murty, Ramachandra V (2016) Process optimization for tannas eproduction by Bacillus gottheilii M2S2 on inert polyurethane foam support. Biocatalysis and Agricultural Biotechnology, 7. pp. 48-55. ISSN 1878-8181

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Abstract

Tanninacylhydrolase(E.C.3.1.1.20) , commonly referred as “tannase”, breaks hydrolysable tannins into gallic acid and glucose.It finds commercial applications in food industry to reduce the level of tanninsin fruit juices,preparation of instant aneous tea and production of gallicacid. In this work, atannasepro- ducing Bacillus gottheilii M2S2 was isolated from the tanneryef fluent soil and asequential optimization strategy was used to determine the effect of carbon source and inducer tannicacid, nitrogen source NH4NO3, inoculumvolume, initial pH, incubation time and mineralsalts(KH2PO4, MgSO4, NaCland CaCl2 � 2H2O) in the production of tannase from Bacillus gottheilii M2S2 in solid state fermentation. Polyure thane foam was used as an inert support for growth of themicroorganism.Themicroorganism was fermentedat32 °C in an optimized liquid medium composedoftannicacid(4%),NH4NO3 (2%), KH2PO4 (0.1%),MgSO4 (0.2%), NaCl(0.1%)andCaCl2 � 2H2O (0.05%)adjustedtoinitialmediapH4.74then inoculated with7.035%vv�1 inoculum and impregnatedonpolyurethanefoamfor26.45hunderstatic condition .A final activityof49.3270.30 UL�1 was achieved,which represents an increase of 328% in relation to the initial unoptimized conditions

Item Type: Article
Uncontrolled Keywords: Tannase Polyurethanefoam Bacillus gottheilii M2S2 Optimization Operating conditions Response-surfacemethodology
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 03 Aug 2016 14:20
Last Modified: 03 Aug 2016 14:20
URI: http://eprints.manipal.edu/id/eprint/146746

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