Store-Operated Calcium Entry through Orai Is Required for Transcriptional Maturation of the Flight Circuit in Drosophila

Pathak, Trayambak (2015) Store-Operated Calcium Entry through Orai Is Required for Transcriptional Maturation of the Flight Circuit in Drosophila. The Journal of Neuroscience, 35 (40). 13784 -13799.

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Abstract

Store operated calcium entry (SOCE) is thought to primarily regulate calcium homeostasis in neurons. Subsequent to identification of Orai as the SOCE channel in nonexcitable cells, investigation of Orai function in neurons demonstrated a requirement for SOCE in Drosophila flight. Here, by analysis of an Orai mutant and by controlled expression of a dominant-negative Drosophila Orai transgene, we show that Orai- ediated SOCE is required in dopaminergic interneurons of the flight circuit during pupal development. Expression of dominant-negative Orai in dopaminergic neurons of pupae abolished flight. The loss of Orai-mediated SOCE alters transcriptional regulation of dopaminergic neurons, leading to downregulation of the enzyme tyrosine hydroxylase, which is essential for dopamine synthesis, and the dopamine transporter, which is required for dopamine uptake after synaptic release. These studies suggest that modulation of SOCE could serve as a novel mechanism for restoring dopamine levels in dopaminergic neurons.

Item Type: Article
Uncontrolled Keywords: Dopamine transporter; Dopaminergic neurons; IP3R; STIM; Tyrosine hydroxylase
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 05 Aug 2016 15:16
Last Modified: 05 Aug 2016 15:16
URI: http://eprints.manipal.edu/id/eprint/146753

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