Store-independent Modulation of Ca2þ Entry Through Orai by Septin 7

Pathak, Trayambak (2016) Store-independent Modulation of Ca2þ Entry Through Orai by Septin 7. Nature Communications. pp. 1-13. ISSN 2041-1723

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Abstract

Orai channels are required for store-operated Ca2þ entry (SOCE) in multiple cell types.Septins are a class of GTP-binding proteins that function as diffusion barriers in cells. Here we show that Septin 7 acts as a ‘molecular brake’ on activation of Orai channels in Drosophila neurons. Lowering Septin 7 levels results in dOrai-mediated Ca2þ entry and higher cytosolic Ca2þ in resting neurons. This Ca2þ entry is independent of depletion of endoplasmic reticulum Ca2þ stores and Ca2þ release through the inositol-1,4,5-trisphosphate receptor. Importantly, store-independent Ca2þ entry through Orai compensates for reduced SOCE in the Drosophila flight circuit. Moreover, overexpression of Septin 7 reduces both SOCE and flight duration, supporting its role as a negative regulator of Orai channel function in vivo.Septin 7 levels in neurons can, therefore, alter neural circuit function by modulating Orai function and Ca2þ homeostasis.

Item Type: Article
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 05 Aug 2016 15:20
Last Modified: 05 Aug 2016 15:20
URI: http://eprints.manipal.edu/id/eprint/146758

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