Ethambutol Induces Testicular Damage and Decreases the Spermfunctional Competence in Swiss Albino Mice

Rao, Arpitha and Nayak, Guruprasad and Kumari, Sandhya and Prabhu, Amratha D and Khandige, Nalini and Kalthur, Sneha Guruprasad and Mutalik, Srinivas and Kalthur, Guruprasad and Adiga, Satish Kumar (2016) Ethambutol Induces Testicular Damage and Decreases the Spermfunctional Competence in Swiss Albino Mice. Environmental Toxicology and Pharmacology, 47. pp. 28-37.

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Abstract

The present study reports the effect of ethambutol (EMB) on testicular function. Prepubertal and adultmale Swiss albino mice were treated with 40, 80, 160 mg/kg body weight of EMB, intraperitoneally,every alternate day for 4 weeks. After 2 weeks gap, mice were sacrificed to collect caudal spermatozoa.EMB treatment resulted in a dose-dependent decrease in the testicular weight, sperm count and motil-ity while the percentage of sperm with head abnormalities, immature chromatin (P < 0.001) and DNAdamage increased (P < 0.01). In addition, EMB treatment resulted in significant depletion of glutathione(P < 0.05–P < 0.01) and histopathological abnormalities such as large cells, vacuolation of tubules and iso-lated colonies of spermatogenic cells were observed. Oct4, 17�-Hsd and c-Kit mRNA was marginallyelevated in EMB treated testes at the highest dose studied. In conclusion, the result of the present studyindicates that EMB has adverse effect on testicular function and impairs the sperm functional competence.

Item Type: Article
Uncontrolled Keywords: Ethambutol;Testicular toxicity; Sperm DNA damage; Testosterone; GSHa
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > KMC Manipal > Biochemistry
Medicine > KMC Manipal > Obstetrics & Gynaecology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 07 Sep 2016 16:02
Last Modified: 07 Sep 2016 16:02
URI: http://eprints.manipal.edu/id/eprint/146931

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