Release of Diclofenac Sodium from Crosslinked Chitosan based Hydrogels: A Comparative Study

Nayak, Ramyasri R and Philip, Jeethu Anna and Dathathri, Eshwari and Thakur, Goutam and Koteshwara, KB (2016) Release of Diclofenac Sodium from Crosslinked Chitosan based Hydrogels: A Comparative Study. In: APA International Conference on Advanced Polymers, Biomaterials,Bioengineering & Nano Drug Delivery (APA-2016), 05/09/2016, Flic-En-Flac, Mauritius.

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Hydrogels are vastly used in tissue engineering and drug-delivery due to their unique properties such as biocompatibility and fluid retention capacity, which enable in developing a sustained release system for rugs/bioactive agents. A comparative study was performed to analyze the release of diclofenac sodium from chitosan based hydrogels using biopolymers like chitosan and gelatin; natural cross-linker like genipin was used to illustrate its effects on gels. Hydrogel composites of chitosan-gelatin in the ratio 1.5:1 were formulated. Diclofenac sodium of 1 %( w/w) was the model drug loaded into hydrogels. Cross-linking was performed with 1% (w/v) genipin solution. Characterization of hydrogels was done using compression test by determining the compressive modulus; swelling test involved immersing hydrogels in water, acidic and basic buffer solutions and calculating swelling indices. Infrared spectroscopy of hydrogels was done to assess the presence of cross-linking. In-vitro release of diclofenac sodium from the chitosan-gelatin hydrogels was done in deionized water for a period of 24hrs; samples were analyzed at 276nm in UV spectrophotometer and cumulative release percentage recorded. All the experiments were done in triplicate (n=3). Results of compression test indicate higher compressive modulus of crosslinked chitosan-gelatin hydrogel than uncross-linked hydrogels. Swelling index was observed to be highest in water. Cross-linked composite hydrogel in water demonstrated lower swelling than that of uncross-linked gels. IR spectroscopy indicated significant absorbance peaks showing presence of cross-linking. Release study of chitosan-gelatin hydrogel without cross-linking showed sustained release of about 30% for a period of 24hrs. Cross-linking of the gels exhibited further promise in modulating release profile of diclofenac sodium. Conclusively, composite cross-linked hydrogels has better stability and can withstand greater stress than uncross-linked gels. Composite hydrogels cross-linked with genipin offered better swelling indices. Sustained release of drug from composite hydrogels was observed owing to their increased viscosity. Cross-linking composite hydrogels can further lower rate of release. Overall, cross-linked gels showed promise as controlled release vehicles for the release of bioactive molecules.

Item Type: Conference or Workshop Item (Paper)
Subjects: Engineering > MIT Manipal > Biomedical
Depositing User: MIT Library
Date Deposited: 20 Sep 2016 15:13
Last Modified: 20 Sep 2016 15:13

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