Advanced Glycation End Products (AGE) potently induce autophagy through activation of RAF protein kinase and nuclear factor KB (NF-KB)

Verma, Neeharika (2016) Advanced Glycation End Products (AGE) potently induce autophagy through activation of RAF protein kinase and nuclear factor KB (NF-KB). Jounal of Biological Chemistry, 291 (3). pp. 1481-1491.

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Abstract

Advanced glycation end products (AGE) accumulate in diabetic patients and aging people because of high amounts of three- or four-carbon sugars derived from glucose, thereby causing multiple consequences, including inflammation, apoptosis, obesity, and age-related disorders. It is important to understand the mechanism of AGE-mediated signaling leading to the activation of autophagy (self-eating) that might result in obesity. We detected AGE as one of the potent inducers of autophagy compared with doxorubicin and TNF. AGE-mediated autophagy is inhibited by suppression of PI3K and potentiated by the autophagosome maturation blocker bafilomycin. It increases autophagy in different cell types, and that correlates with the expression of its receptor, receptor for AGE. LC3B, the marker for autophagosomes, is shown to increase upon AGE stimulation. AGE-mediated autophagy is partially suppressed by inhibitor of NF-κB, PKC, or ERK alone and significantly in combination. AGE increases sterol regulatory element binding protein activity, which leads to an increase in lipogenesis. Although AGE-mediated lipogenesis is affected by autophagy inhibitors, AGE-mediated autophagy is not influenced by lipogenesis inhibitors, suggesting that the turnover of lipid droplets overcomes the autophagic clearance. For the first time, we provide data showing that AGE induces several cell signaling cascades, like NF-κB, PKC, ERK, and MAPK, that are involved in autophagy and simultaneously help with the accumulation of lipid droplets that are not cleared effectively by autophagy, therefore causing obesity.

Item Type: Article
Uncontrolled Keywords: NF-κB transcription factor; autophagy; diabetes; lipid droplet; obesity; signal transduction.
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 04 Oct 2016 15:51
Last Modified: 04 Oct 2016 15:51
URI: http://eprints.manipal.edu/id/eprint/146999

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