Smaller to Larger Biomolecule Detection using a Lab-built Surface Plasmon Resonance based Instrument

Lukose, J and Kulal, V and Chidangil, Santhosh and Sinha, RK (2016) Smaller to Larger Biomolecule Detection using a Lab-built Surface Plasmon Resonance based Instrument. Laser Physics, 26. pp. 1-8. ISSN 1555-6611

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Abstract

We have developed a low-cost surface plasmon resonance (SPR) instrument based on the Kretschmann configuration for biosensing applications. The fabricated instrument is capable of operating in both angular and intensity interrogation schemes. The proposed sensor has proved enormously versatile by detecting a range of analytes with low to high molecular weights. The refractive index based sensor has been used for detecting the variation in the concentration of the aqueous solution of glucose and glycerine. Real time immobilization of protein molecules, bovine serum albumin on a gold (Au) film surface, has also been detected using the SPR imaging technique. Alkanethiol functionalization of the Au surface was performed, and bovine serum albumin was immobilized onto the carboxyl functionalized surface using amine reactive cross linker chemistry. In future, the present approach can also be utilized for the selective detection of a wide range of target biomolecules with the help of specific capture probes, as well as for monitoring protein–drug interactions.

Item Type: Article
Uncontrolled Keywords: Surface plasmon resonance; Glucose; Glycerine; Bovine serum albumin; Self assembled monolayer
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Library
Date Deposited: 04 Oct 2016 14:06
Last Modified: 04 Oct 2016 14:06
URI: http://eprints.manipal.edu/id/eprint/147075

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