Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla

Swathy, SP and Kodsara, Ramachandra Kiran and Rao, MS and Mahato, KK and Rao, M and Satyamoorthy, K and Muthusamy, A (2016) Responses of He-Ne laser irradiation on agronomical characters and chlorogenic acid content of brinjal (Solanum melongena L.) var. Mattu Gulla. Journal of Photochemistry & Photobiology, B: Biology, 164. pp. 182-190.

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Abstract

Exposure to laser irradiation on seeds brings about the changes in agronomical characteristics of the plants. Solanum melongena L. var. Mattu Gulla, a variety of brinjal is of high economic value due to its unique colour and flavour. The aim of the study was to understand the influence of Helium-Neon (He-Ne) laser irradiation on agronomical characters of Solanum melongena L. var.Mattu Gulla in the field conditions. Various growth characteristics including seed germination percentage, survival rate, plant height, number of branches, and flowers and fruits were estimated during different developmental stages of the brinjal. In addition, the chlorogenic acid content of fruits obtained from the laser irradiated seeds were quantified using reversed-phase high-performance liquid chromatography (RP- HPLC). The plants from the seeds irradiated with different doses (20, 25 and 30 J/ cm2) of He-Ne laser showed significant enhancement on the growth characteristics when compared to the non-irradiated control groups. He-Ne laser irradiation also improved the yield characteristics of the plants significantly in in vivo conditions in comparison with control group. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed using methanolic extract of matured fruit ofMattu Gulla on HepG2 and fibroblast cell lines. The IC50 values of fruit extract from laser irradiated groups were found to be similar to non-irradiated control groups. Chlorogenic acid content was found to be higher in 20 J/cm2 and lower in 30 J/ cm2 treated fruit tissue. The current study thus elucidates the role of He-Ne laser as a biostimulator on brinjal var. Mattu Gulla not only in the in vitro conditions but also in the in vivo field conditions.

Item Type: Article
Uncontrolled Keywords: Solanum melongena L; Mattu Gulla seeds; He-Ne laser; Chlorogenic acid; RP-HPLC; MTT assay.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 16 Nov 2016 12:00
Last Modified: 16 Nov 2016 12:00
URI: http://eprints.manipal.edu/id/eprint/147530

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