The Prognostic Implication of the Expression of EGFR, p53, cyclin D1, Bcl-2 and p16 in Primary Locally advanced Oral Squamous Cell Carcinoma Cases: A Tissue Microarray Study

Solomon, Monica Charlotte and Vidyasagar, MS and Fernandes, Donald J and Guddattu, Vasudeva and Mathew, Mary and Shergill, Ankur Kaur and Carnelio, Sunitha and Chandrashekar, Chetana (2016) The Prognostic Implication of the Expression of EGFR, p53, cyclin D1, Bcl-2 and p16 in Primary Locally advanced Oral Squamous Cell Carcinoma Cases: A Tissue Microarray Study. Medical Oncology, 33 (12). pp. 1-7. ISSN 1357-0560

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Abstract

Oral squamous cell carcinomas comprise a heterogeneous tumor cell population with varied molecular characteristics, which makes prognostication of these tumors a complex and challenging issue. Thus, molecular profiling of these tumors is advantageous for an accurate prognostication and treatment planning. This is a retrospective study on a cohort of primary locally advanced oral squamous cell carcinomas (n = 178) of an Indian rural population. The expression of EGFR, p53, cyclin D1, Bcl-2 and p16 in a cohort of primary locally advanced oral squamous cell carcinomas was evaluated. A potential biomarker that can predict the tumor response to treatment was identified. Formalin- fixed paraffin-embedded tumor blocks of (n = 178) of histopathologically diagnosed cases of locally advanced oral squamous cell carcinomas were selected. Tissue microarray blocks were constructed with 2 cores of 2 mm diameter from each tumor block. Four-micron-thick sections were cut from these tissue microarray blocks. These tissue microarray sections were immunohistochemically stained for EGFR, p53, Bcl-2, cyclin D1 and p16. In this cohort,EGFR was the most frequently expressed 150/178 (84%) biomarker of the cases. Kaplan–Meier analysis showed a significant association (p = 0.038) between expression of p53 and a poor prognosis. A Poisson regression analysis showed that tumors that expressed p53 had a two times greater chance of recurrence (unadjusted IRR—95% CI 2.08 (1.03, 4.5), adjusted IRR—2.29 (1.08, 4.8) compared with the tumors that did not express this biomarker. Molecular profiling of oral squamous cell carcinomas will enable us to categorize our patients into more realistic risk groups. With biologically guided tumor characterization, personalized treatment protocols can be designed for individual patients,which will improve the quality of life of these patients.

Item Type: Article
Uncontrolled Keywords: Biomarkers; Prognostication; Tissue microarray; Carcinomas; Recurrence; Treatment
Subjects: Dentistry > MCODS Manipal > Oral Pathology and Microbiology
Departments at MU > Statistics
Medicine > KMC Manipal > Pathology
Medicine > KMC Manipal > Radiotherapy and Oncology
Depositing User: KMC Library
Date Deposited: 17 Dec 2016 08:43
Last Modified: 17 Dec 2016 08:43
URI: http://eprints.manipal.edu/id/eprint/147738

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