Antioxidant and Nephroprotective Activities of the Extract and Fractions of Homonoia riparia Lour

Xavier, Seena Kanniparambil and Shoja, Muhammed Haneefa and Devkar, Raviraj Anand and Rao, Picheswara P and Maheshwari, Rajalekshmi and Shastry, Chandrashekara Shreedhara and Setty, Manjunath M (2017) Antioxidant and Nephroprotective Activities of the Extract and Fractions of Homonoia riparia Lour. Pharmacognosy Magazine, 13 (49). pp. 25-30. ISSN 0973-1296

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Abstract

Background: Homonoia riparia is a plant, which is widely used in the indigenous system of medicine for the treatment of urolithiasis, renal disorders and inflammatory conditions. This is the first report on the antioxidant and nephroprotective activities of whole plant of H. riparia. Objective: The present study aims at investigating the in vitro antioxidant and nephroprotective activity of the methanol extract and its different fractions of H. riparia. Methods: Petroleum ether (HRPE), Ethyl acetate (HREA), Butanol (HRBU), aqueous fractions (HRAQ) were prepared from the crude methanol extract of H. riparia (HRM) using liquid partitioning.Total phenolic content, flavonoid content and antioxidant activity assay were performed according to suitable methods. Nephroprotective activities were evaluated by MTT assay using Human Embryonic Kidney cells against cisplatin induced toxicity. Quantification of gallic acid was performed using validated HPTLC method.Results: The studies showed that extract and fractions possess significant nephroprotective activity against cisplatin induced renal toxicity. All the extracts/fractions of whole plant of Homonoia riparia was found to be significantly reducing cisplatin induced toxicity (< 0.05). The highest activity was observed with HRBU and HRAQ with a percentage viability of 293.09 ± 4.3 and 345.07 ± 3.2 at a concentration of 200 μg/ml. Gallic acid was detected in the HRM/fractions using HPTLC

Item Type: Article
Uncontrolled Keywords: Antioxidant activity; Gallic acid; Homonoia riparia; HEK 293; Nephroprotective
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 11 Jan 2017 13:38
Last Modified: 11 Jan 2017 13:38
URI: http://eprints.manipal.edu/id/eprint/147988

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