Biosynthesis of Copper Nanoparticles using Copper-resistant Bacilluscereus, a Soil Isolate

Tiwari, Mradul and Jain, Prateek and Raghu, Chandrasekhar H and Kasinathan, Narayanan and Udupa, N and Rao, Venkata J (2016) Biosynthesis of Copper Nanoparticles using Copper-resistant Bacilluscereus, a Soil Isolate. Process Biochemistry, 51 (10). pp. 1348-1356. ISSN 1359-5113

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Abstract

Microorganisms are useful systems for the production of biocompatible metal nanoparticles. Copper, anessential element of life, has good therapeutic potential. However, copper lacks suitable form for effectivein vivo delivery, which has diminished its applicability. In this study, we produced biosynthesized coppernanoparticles (BCuNps) using a copper-resistant bacterial isolate from copper mine. The organism wasable to tolerate >10 mM of copper and when analysed by 16S rRNA technique, showed 100% similaritywith Bacillus cereus. BCuNps, produced by this microorganism, in cell-free filtrate, were characterized forsurface plasmon resonance (SPR), particle’s characteristics, spectroscopic properties and morphology.SPR peaks for BCuNps were recorded between 570–620 and 350–370 nm. BCuNps characteristics, namelyparticle size distribution, polydispersity index and zeta potential were found to be 11–33 nm, 0.433 and(−) 19.6 mV, respectively. Scanning electron microscope (SEM), transmission electron microscope (TEM)and atomic force microscope (AFM) analyses confirmed the uniform morphology; X- ray diffraction (XRD)spectrum revealed the crystalline nature; and Fourier transform infrared (FTIR) spectrum disclosed thepresence of protein with BCuNps. A comparative evaluation of BCuNps with copper sulphate to determinetheir antimicrobial and cell toxicity levels was undertaken. BCuNps showed better antimicrobial effectand found to be safer against normal cell lines, such as HaCat, Vero and hFOB, than the copper sulphatecontrol.

Item Type: Article
Uncontrolled Keywords: Biosynthesis; Characterization; Copper resistance; Copper nanoparticles; Microbial screening; Nanobiotechnologya
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 12 Jan 2017 15:31
Last Modified: 12 Jan 2017 15:31
URI: http://eprints.manipal.edu/id/eprint/148038

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