Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres

Kotian, Ravindra and Madhyastha, Prashanthi S and Shobha, KL and Rao, Satish BS and Ginjupalli, Kishore (2017) Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres. World Journal of Dentistry, 8 (1). pp. 28-36. ISSN 976-6006

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Abstract

Aim: This study was performed to evaluate the biocompatibility of pure titanium and Ti-6Al-4V metals coated with hydroxyapatite (HA) by plasma spray using different plasma gas atmospheres.Materials and methods: The cell viabilities for each HA-coated sample in an atmosphere of argon, argon–hydrogen, nitrogen,and nitrogen–hydrogen were studied using MTT assay and platelet adhesion test.Results: The mean cell viabilities by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assay of samples coated with HA in argon–hydrogen plasma atmosphere showed maximum cell viability at different time intervals compared with other coating atmospheres of argon–hydrogen, nitrogen, and nitrogen– hydrogen. A statistically significant value of cell viability (p < 0.001) was observed between and within the groups of argon,argon–hydrogen, nitrogen, and nitrogen–hydrogen plasma gas atmosphere. The platelet adhesion study showed agglomerates of platelet cells in some isolated regions of HA for all atmospheres.Significance: The results obtained in this study can serve as a guide for the development of new Ti-based HA-coated implants in different plasma gas atmospheres.

Item Type: Article
Uncontrolled Keywords: Cytocompatibility; Hydroxyapatite; MTT assay; Plasma coating; Platelet adhesion; Titanium; Ti-6Al-4V
Subjects: Dentistry > MCODS Manipal > Dental Materials
Life Sciences > MLSC Manipal
Medicine > MMMC Manipal > Microbiology
Depositing User: KMC Library
Date Deposited: 14 Jan 2017 14:55
Last Modified: 14 Jan 2017 14:55
URI: http://eprints.manipal.edu/id/eprint/148071

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