Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

Rao, Pragna (2016) Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus. Intractable & Rare Diseases Research, 5 (1). pp. 25-30.

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Abstract

Type 2 diabetes mellitus (T2DM) and post-transplant diabetes mellitus (PTDM) are nonsynonymousforms of diabetes. Glucokinase (GCK) plays a key role in glucose metabolism. The relationship between the GCK promoter and specific types of diabetes, such as PTDM and T2DM, in the Asian Indian population is unknown. We examined the occurrence of a specific GCK promoter variant (-258G/A) in patients with T2DM and PTDM. The casecontrol study enrolled 640 Asian Indian subjects, including controls (n = 250) and T2DM (n = 250), PTDM (n = 42), and non-post-transplant diabetes mellitus (non-PTDM) (n = 98) patients. Purified Deoxyribonucleic acid (DNA) was genotyped with the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. The digested PCR products were analyzed on 12% polyacrylamide gels. The anthropometric, biochemical, and clinical details of each group were documented. GCK -258G/A alleles and genotypes were not associated with T2DM. However, among PTDM subjects, we detected a higher frequency of heterozygotes (52.4%) and a positive association with alleles/genotypes. The results suggest that the promoter region (-258G/A) of GCK plays an important role in PTDM in Asian Indians.

Item Type: Article
Uncontrolled Keywords: GCK; -258G/A; T2DM; PTDM; Asian Indians.
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Library
Date Deposited: 14 Mar 2017 03:40
Last Modified: 14 Mar 2017 03:40
URI: http://eprints.manipal.edu/id/eprint/148431

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