MU Digital Repository
Logo

Comparison of Immunofluorescence and Desmoglein Enzyme‑linked Immunosorbent Assay in the Diagnosis of Pemphigus: A Prospective,Cross‑sectional Study in a Tertiary Care Hospital

Deepthi, Ravi and Prabhu, Smitha S and Rao, Raghavendra and Balachandran, C and Bairy, Indira (2017) Comparison of Immunofluorescence and Desmoglein Enzyme‑linked Immunosorbent Assay in the Diagnosis of Pemphigus: A Prospective,Cross‑sectional Study in a Tertiary Care Hospital. Indian Journal of Dermatology, 62 (2). pp. 171-177. ISSN 0019-5154

[img] PDF
2209 DisplayPdf.pdf - Published Version
Restricted to Registered users only

Download (2MB) | Request a copy

Abstract

Background: Pemphigus is an acquired immunobullous disorder in which antibodies are directed against epidermal cadherins. Despite the commercial availability and less cost of enzyme‑linked immunosorbent assays (ELISAs) to detect antidesmoglein 1 (Dsg1) and anti-Dsg3, immunofluorescence is still widely used for confirmation of diagnosis. Aims: (1) To compare the usefulness of indirect immunofluorescence (IIF) and ELISA tests in the diagnosis of pemphigus. (2) To find the clinical correlation between the tests and severity of the disease. Materials and Methods: Sixty‑one patients (27 women and 34 men, age distribution from 20 to 75) were clinically diagnosed as pemphigus (pemphigus foliaceus ‑ 11, pemphigus vulgaris ‑ 50) and were recruited for the study.IIF and Dsg ELISA were performed and the findings were compared with each other and with the pemphigus area activity score. Data were entered in SPSS and were analyzed using Kruskal–Wallis test. Results: There was a moderate positive correlation between the cutaneous score and Dsg1 titer,and mucosal score and Dsg3 titer. The titer of IIF showed statistically significant positive correlation with the cutaneous score but not the mucosal score. Dsg ELISA showed higher sensitivity (90.2%) than IIF (75.4%) in the diagnosis of pemphigus. Conclusions: Dsg ELISA is a more sensitive method than IIF and shows more correlation with the disease severity.

Item Type: Article
Uncontrolled Keywords: Desmoglein enzyme‑linked immunosorbent assay; Indirect immunofluorescence; Pemphigus
Subjects: Medicine > KMC Manipal > Dermatology
Medicine > KMC Manipal > Microbiology
Depositing User: KMC Library
Date Deposited: 20 Mar 2017 03:56
Last Modified: 20 Mar 2017 03:56
URI: http://eprints.manipal.edu/id/eprint/148523

Actions (login required)

View Item View Item