Influence of peptide dendrimers and sonophoresis on the transdermal delivery of ketoprofen

Manikkath, Jyothsna and Hegde, Aswathi R and Kalthur, Guruprasad and Mutalik, Srinivas (2017) Influence of peptide dendrimers and sonophoresis on the transdermal delivery of ketoprofen. International Journal of Pharmaceutics, 521 (1-2). pp. 110-119. ISSN 0378-5173

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Abstract

The aim of this study was to determine the individual and combined effects of peptide dendrimers and low frequency ultrasound on the transdermal permeation of ketoprofen. Arginine terminated peptide dendrimers of varying charges (4+, 8+ and 16+, named as A4. A8 and A16 respectively) were synthesized and characterized. Ketoprofen was subjected to passive, peptide dendrimer-assisted and sonophoretic permeation studies (with and without dendrimer application) across Swiss albino mouse skin, both in vitro and in vivo. The studies revealed that the synthesized peptide dendrimers considerably increased the transdermal permeation of ketoprofen and displayed enhancement ratios of up to 3.25 (with A16 dendrimer), compared to passive diffusion of drug alone in vitro. Moreover, the combination of peptide dendrimer treatment and ultrasound application worked in synergy and gave enhancement ratios of up to 1369.15 (with ketoprofen-A16 dendrimer complex). In vivo studies demonstrated that dendrimer and ultrasound-assisted permeation of drug achieved much higher plasma concentration of drug, compared to passive diffusion. Comparison of transdermal and oral absorption studies revealed that transdermal administration of ketoprofen with A8 dendrimer showed comparable absorption and plasma drug levels with oral route. The excised mouse skin after in vivo permeation study with dendrimers and ultrasound did not show major toxic reactions. This study demonstrates that arginine terminated peptide dendrimers combined with onophoresis can effectively improve the transdermal permeation of ketoprofen.

Item Type: Article
Uncontrolled Keywords: Transdermal; peptide dendrimers; sonophoresis; ketoprofen; ultrasound.
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 27 Mar 2017 04:27
Last Modified: 27 Mar 2017 04:27
URI: http://eprints.manipal.edu/id/eprint/148575

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