Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models

Nalloor, Thomas JP and Narayanan, Kasinathan and Kumar, Nitesh (2017) Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models. Journal of Basic and Clinical Physiology and Pharmacology, 28 (3). pp. 257-265. ISSN 0792-6855

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Abstract

Background: Butter is one of the widely used fats present in the diet. However, there is no satisfactory study available that evaluates the effect of a high-fat diet containing butter as the principal fat on the development of non-alcoholic fatty liver disease (NAFLD). Methods: In the present study, butter was used for the development of steatosis in Chang liver cells in an in vitro study and Swiss albino mice in an in vivo study. In vitro steatosis was established, and butter was compared with oleic acid in Chang liver cells using an oil red O (ORO)- based colorimetric assay. In the in vivo study, a butter-rich special diet was fed for 15 weeks to mice, who showed no significant change in body weight. The expression pattern of phosphatase and tensin homolog (PTEN) and miR-21 was compared by reverse transcriptase-PCR. Results and Conclusions: Special diet-fed animals showed downregulated PTEN compared to normal diet-fed animals, while levels of miR-21 remained the same. Elevations in biochemical parameters, viz., triglycerides and liver function tests showed symptoms of onset of NAFLD. Histophathological study of livers of test animals confirmed mild-to-moderate degree of NAFLD.

Item Type: Article
Uncontrolled Keywords: Butter; miR-21; non-alcoholic fatty liver disease (NAFLD); non-alcoholic steatohepatitis (NASH); phosphatase and tensin homolog (PTEN); steatosis.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 15 May 2017 11:23
Last Modified: 15 May 2017 11:23
URI: http://eprints.manipal.edu/id/eprint/148867

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