Haploid parthenotes express differential response to in vitro exposure of ammonia compared to normally fertilized embryos

Nair, Ramya and Mutalik, Srinivas and Kalthur, Guruprasad and Adiga, Satish Kumar (2017) Haploid parthenotes express differential response to in vitro exposure of ammonia compared to normally fertilized embryos. Biochemical and Biophysical Research Communications, 486 (1). pp. 1-6. ISSN 1090-2104

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Abstract

In the present study, we assessed whether absence of paternal genome imparts any differential response in embryos to chemical stress such as ammonia. Parthenogenesis was induced in MII stage oocytes using 10 mM SrCl2 in M16 medium. Parthenotes and normally fertilized embryos at 2 cell stage were exposed to different concentrations of ammonia and cultured till blastocyst. Exposure of ammonia to normally fertilized embryos resulted in significant decrease in the developmental potential (p < 0.0001) and blastocyst quality (p < 0.001). Whereas, in parthenotes, even though lower concentrations of ammonia did not have any effect, at 200 mM concentration the blastocyst rate was two times higher than control. The baseline apoptotic index was higher in parthenotes compared to normally fertilized embryos, which further increased after ammonium exposure (p < 0.001). Unlike in normally fertilized embryos ammonia exposure altered the mitochondrial distribution pattern and lead to increased expression of Oct4, Nanog and Naþ/Kþ ion exchange channel, while the cytochrome C expressionwas downregulated. This indicates that haploidy and/or absence of paternal factors in the embryo results in differential tolerance to stress induced by ammonia.

Item Type: Article
Uncontrolled Keywords: Parthenogenesis; ammonia; haploidy; stress response; apoptosis.
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 20 May 2017 09:14
Last Modified: 20 May 2017 09:14
URI: http://eprints.manipal.edu/id/eprint/148924

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