Genotype-phenotype association of TGF-β1 and GST with chemo-radiotherapy induced toxicity

Goutham, Venkatesh Hassan and Mumbrekar, Kamalesh Dattaram and Negi, Hitendra and Fernandes, Donald J and Rao, Satish BS (2017) Genotype-phenotype association of TGF-β1 and GST with chemo-radiotherapy induced toxicity. International Journal of Radiation Research, 15 (1). pp. 15-23.

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Abstract

Background: Normal �ssue toxicity con�nues to remain as a major challenge for radia�on oncologists for delivering the total dose to the tumour cells in cancer pa�ents. Cellular, molecular and plasma based early biomarkers to predict the overreac�ons and non-overreac�ons of normal �ssue toxicity before the ini�a�on of radiotherapy can be valuable for personalised treatment. The aim of the current study was to analyse the interrela�onship between polymorphisms in Glutathione S- Transferases (GSTs) and Transforming Growth Factor-β1 (TGF-ß1), the plasma level/ac�vity of these proteins with the development of chemo-radiotherapy induced oral mucosi�s and skin reac�on in head and neck cancer (HNC) pa�ents. Materials and Methods: We analysed polymorphisms in TGF-β1 and GST by restric�on diges�on of the PCR amplified products and we also assessed circula�ng TGF- β1 levels and GST ac�vity by Enzyme Linked Immunosorbent Assay (ELISA). Results: The results indicate that pre-radiotherapy plasma TGF-β1 levels and total GST ac�vity has no correla�on with radia�on induced normal �ssue skin reac�on and oral mucosi�s in HNC pa�ents. Conclusion: The selected polymorphisms in TGF-β1 and GST had no influence on TGF-β1 levels and total GST ac�vity. Plasma TGF-β1 and GST ac�vity was not affected by the presence of selected polymorphisms and lacks significance in predic�ng skin reac�on and oral mucosi�s prior to chemo-radiotherapy.

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