Expression, functional characterization and x-ray analysis of HosA, a member of MarR family of transcription regulator from uropathogenic escherichia coli

Roy, Ajit and Sawhney, Bhavik and Ghosh, Debasish Kumar (2016) Expression, functional characterization and x-ray analysis of HosA, a member of MarR family of transcription regulator from uropathogenic escherichia coli. The Protein Journal, 35 (4). pp. 269-282.

[img] PDF
The Protein Journal- 2016, Volume 35, Issue 4, pp 269–282.pdf - Published Version
Restricted to Registered users only

Download (6MB) | Request a copy

Abstract

Regulators belonging to multiple antibiotic resistance regulator (MarR) family are widespread in prokaryotes and are involved in regulation of genes that are responsible for virulence and pathogenicity in most of the clinically important pathogens. Here we report the transcriptional, biophysical, and X-ray analyses of homologue of SlyA (HosA), a member of MarR family that is predominantly present in the pathogenic strains of Enterobacteriaceae family. The initiation of hosA transcription was observed to occur at two independent start sites and subsequent binding study has revealed that the purified HosA interacts with its upstream region suggesting a probable autoregulation. The secondary structure analysis through circular dichroism spectroscopy demonstrated that HosA is predominantly composed of the alpha helix with higher thermal stability. To further understand the threedimensional structure, HosA was crystallized and the crystals were diffracted to maximum of 2.9 A ° ´ on exposure to X-rays. Analysis of the X-ray crystallographic data suggested a primitive space group (P 6 ? 2 2), with unit cell parameters a = b = 64.19 A ° and c = 244.25 A ° . The solvent content and Matthews coefficient were 41 % and 2.11 A ° 3 Da-1, respectively, which indicated the existence of two molecules of HosA in the asymmetric unit of crystal.

Item Type: Article
Uncontrolled Keywords: HosA ;MarR;Uropathogenic E. coli strain UMN026 ; mutS-rpoS polymorphism ; X-ray crystallography.
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Manipal
Date Deposited: 25 May 2017 03:50
Last Modified: 25 May 2017 03:50
URI: http://eprints.manipal.edu/id/eprint/148954

Actions (login required)

View Item View Item