Effect of nucleants in photothermally assisted crystallization

Shilpa, T and George, Sajan D and Bankapur, Aseefhali and Chidangil, Santhosh and Mathur, Deepak and Salam, Abdul Ajees Abdul (2017) Effect of nucleants in photothermally assisted crystallization. Photochemical & Photobiological Sciences, 1 (3). pp. 1-23.

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Abstract

Laser-induced crystallization is emerging as a promising technique to crystalize biomolecules like amino acids and proteins. The use of external materials as nucleants and novel seeding methods open new paths for protein crystallization. We report here results of experiments that explore the effect of nucleants on laser-based crystallization of microlitre droplets of small molecules, amino acids, and protein. The role of parameters like solute concentration, droplet volume, type and size of the nucleant, and laser power, are systematically investigated. In addition to crystallization of standard molecules like NaCl, KCl, and glycine, we demonstrate crystallization of negatively (L-histidine), positively (L-aspartic acid) charged amino acids and lysozyme protein. Single crystal X-ray diffraction and Raman spectroscopy studies unequivocally indicate that the nucleants do not alter the molecular structure of glycine, the hydrogen bond patterns, and packing. Localized vaporization of the solvent near the nucleant due to photothermal heating has enabled us to achieve rapid crystallization - within 3 s - at laser intensities of 0.1 MW/cm2 , significantly lower than those reported earlier, with both saturated and unsaturated solutions. The outcome of the current experiments may be of utility in tackling various crystallization problems of forming crystals large enough to perform X-ray crystallography.

Item Type: Article
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Library
Date Deposited: 08 Jun 2017 05:02
Last Modified: 08 Jun 2017 05:02
URI: http://eprints.manipal.edu/id/eprint/149010

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