Pinocembrin enriched fraction of Elytranthe parasitica (L.) Danser induces apoptosis in HCT 116 colorectal cancer cells

Kumar, Nimmy and Biswas, Subhankar and Shrungeswara, Akhila Hosur and Mallik, Sanchari Basu and Mathew, Jessy Elizabeth and Mathew, Jesil A and Nandakumar, K and Lobo, Richard (2017) Pinocembrin enriched fraction of Elytranthe parasitica (L.) Danser induces apoptosis in HCT 116 colorectal cancer cells. Journal of Infection and Chemotherapy, 23 (6). pp. 354-359.

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Abstract

Objective: The goal of the present study was to assess the antiproliferative and apoptosis-inducing effects of stem parts of Elytranthe parasitica (L.) Danser (EP) on colorectal cancer and identify the bioactive phytochemicals. Material and methods :EP methanol extract (EP.M) and its subsequent fractions were screened for antiproliferative activity in human colorectal carcinoma HCT 116 cell line. Phytocomposition of the bioactive fraction was analyzed by GC–MS. Further, apoptotic induction and cell cycle arrest was assessed in the most bioactive fractions. Results :EP.DEE (Diethyl Ether) fraction and a subsequent fraction derived by column chromatography, Fraction 3A (FR 3A) significantly inhibited the proliferation of HCT 116 cells (P < 0.05). FR 3A triggered apoptosis and notably modulated the cell cycle checkpoints. GC–MS analysis of FR 3A revealed the presence of 24 phytochemicals, the most prominent of which was pinocembrin (70.67%), a flavonoid. Conclusion:Hence, it could be speculated that pinocembrin and its related derivatives may be the chief phytochemicals involved in apoptosis – mediated cytotoxicity of the enriched fraction. Our findings indicate the enriched fraction is a promising candidate which could be developed into a natural chemotherapeutic product for colorectal cancer therapy.

Item Type: Article
Uncontrolled Keywords: Apoptosis; Colorectal carcinoma; Cell cycle; HCT 116; Pinocembrin.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmacognosy
Pharmacy > MCOPS Manipal > Pharmacology
Pharmacy > MCOPS Manipal > Pharmacy Practice
Depositing User: KMC Library
Date Deposited: 10 Jun 2017 03:39
Last Modified: 10 Jun 2017 03:39
URI: http://eprints.manipal.edu/id/eprint/149022

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