Effect of insulin on spatial memory in aluminum chloride-induced dementia in rats

Madhavan, Nampoothiri and Nitesh, Kumar and Grandhi, Venkata Ramalingayya and Kutty, Gopalan N and Nandakumar, Krishnadas and Rao, Mallikarjuna C (2017) Effect of insulin on spatial memory in aluminum chloride-induced dementia in rats. Neuroreport, 28 (9). pp. 540-544. ISSN 0959-4965

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Abstract

Latest reports suggest the involvement of insulin in modulating memory. A few published in-vitro studies favor the antidementia effect of insulin. Thus, the present study aimed to evaluate the prophylactic role of insulin and its combination with glucose and its possible mechanism(s) in an aluminum chloride (AlCl3)-induced cognitive dysfunction model in rodents, with a special focus on memory centers namely, the hippocampus and the frontal cortex. Male Wistar rats were exposed to AlCl3 (175 mg/kg orally) for 60 days. Insulin (0.5 IU/kg), Insulin (0.5 IU/kg) in combination with glucose (200 mg/kg), and rivastigmine (1 mg/kg) were administered intraperitoneally 45 min before the administration of AlCl3 for 60 days. Spatial memory was assessed using the Morris water-maze test. After 60 days of treatment, animals were killed, and the hippocampus and frontal cortex were collected and analyzed for acetylcholinesterase activity and antioxidant enzyme level. Blood glucose levels were also analyzed. Treatment with the standard drug, rivastigmine (1 mg/kg), produced a significant reduction in escape latency and increased the time spent in the target quadrant compared with the AlCl3-treated group. Insulin and its combination with glucose could not inhibit the behavioral impairments in aluminum-exposed rats. Treatment with insulin alone and its combination with glucose reversed the increased glucose levels. Insulin alone and its combination with glucose could not inhibit aluminum-induced oxidative stress and impaired cholinergic transmission in the hippocampus and frontal cortex regions. The study suggests the inability of prophylactic insulin administration against cognitive dysfunction induced by environmental toxin (AlCl3) in the hippocampus and the frontal cortex.

Item Type: Article
Uncontrolled Keywords: Acetylcholinesterase; aluminum chloride; glucose; insulin; memory; Morris water maze.
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 10 Jun 2017 03:50
Last Modified: 10 Jun 2017 03:50
URI: http://eprints.manipal.edu/id/eprint/149026

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