Insulin protects against brain oxidative stress with an apparent effect on episodic memory in doxorubicin-induced cognitive dysfunction in wistar rats

Ramalingayya, Grandhi Venkata and Sonawane, Vishwajeet and Cheruku, Sri Pragnya and Kishore, Anoop and Nayak, Pawan G and Kumar, Nitesh and Shenoy, Rekha R and Nandakumar, Krishnadas (2017) Insulin protects against brain oxidative stress with an apparent effect on episodic memory in doxorubicin-induced cognitive dysfunction in wistar rats. Journal of Environmental Pathology, Toxicology and Oncology, 36 (2). pp. 121-130. ISSN 0731-8898

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Abstract

The present study was aimed at assessing the protective effect of insulin against doxorubicin (DOX) -induced cognitive dysfunction in Wistar rats. Cognitive function for episodic memory was assessed by a novel object recognition task (NORT) in male Wistar rats. Oxidative stress markers—SOD, catalase, glutathione, and lipid peroxidation—in the hippocampus and frontal cortex were assessed using colorimetric methods. Doxorubicin treatment (2.5 mg/kg, i.p., every 5 days for 50 days) reduced recognition and discriminative indices in NORT with increased oxidative stress in the brain. A nonhypoglycemic dose of insulin (0.5 IU/kg, i.p.) significantly reduced brain oxidative stress (MDA) induced by doxorubicin with an increase in the antioxidant defense systems (SOD, catalase, and GSH). Rats treated with combined insulin and DOX spent comparatively more time with the novel object when compared to the non-novel objects; however, the observed difference was not statistically significant. An apparent improvement (p < 0.26) in recognition of the novel object was observed against the damage induced by doxorubicin. These results suggest that insulin reduces brain oxidative stress and apparently improves doxorubicin-induced cognitive dysfunction in Wistar rats.

Item Type: Article
Uncontrolled Keywords: chemobrain; cognitive function; doxorubicin; novel object recognition task; oxidative stress.
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 29 Jun 2017 04:57
Last Modified: 29 Jun 2017 04:57
URI: http://eprints.manipal.edu/id/eprint/149214

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