Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP–HPLC method

Managuli, Renuka S and Gourishetti, Karthik and Shenoy, Rekha R and Koteshwara, Kunnatur B and Reddy, Meka Sreenivasa and Mutalik, Srinivas (2017) Preclinical pharmacokinetics and biodistribution studies of asenapine maleate using novel and sensitive RP–HPLC method. Bioanalysis, 9 (14). pp. 1037-1047. ISSN 1757-6180

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Abstract

Aim: Asenapine maleate (ASPM) is a newer antipsychotic drug available as a sublingual tablet in the market. Experimental: To investigate the pharmacokinetic and tissue distribution study of ASPM following oral administration in rats, reversed-phase HPLC method was developed and validated. Results: ASPM was extracted from plasma and tissue matrix by liquid–liquid extraction technique and analyzed using mobile phase consisted of phosphate buffer pH 3.0 and acetonitrile (65:35% v/v). The method showed good linearity (10–500 ng/ml) with recovery 83–102%. In pharmacokinetics study, half-life was 32.74 ± 7.51 h due to slow elimination of drug. The biodistribution study indicated preferential distribution of ASPM to highly perfused organs. Conclusion: The current method can be successfully applied for estimating the drug in various biological matrices.

Item Type: Article
Uncontrolled Keywords: Asenapine maleate ; liquid–liquid extraction ; pharmacokinetic ; RP–HPLC ; tissue distribution.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutics
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 12 Aug 2017 03:39
Last Modified: 12 Aug 2017 03:39
URI: http://eprints.manipal.edu/id/eprint/149484

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