Oral candidal speciation, virulence and antifungal susceptibility in type 2 diabetes mellitus

Patel, Pratik Nalinbhai and Sah, Parul and Chandrashekar, Chetana and Vidyasagar, Sudha and Rao, Venkata J and Tiwari, Mradul and Radhakrishnan, Raghu (2017) Oral candidal speciation, virulence and antifungal susceptibility in type 2 diabetes mellitus. Diabetes Research and Clinical Practice, 125. pp. 10-19. ISSN 0168-8227

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Objectives: To determine the oral candidal carriage (OCC), activity of virulent factors and fluconazole susceptibility in subjects with type 2 diabetes mellitus (T2DM) and investigate their association with HbA1c measurements. Materials and methods: A cross sectional study was conducted on 100 diabetics and 100 healthy volunteers. The virulence was assessed by measuring the phospholipase activity and proteolysis index. Fluconazole susceptibility was performed using the gradient diffusion method. The OCC, virulence factors and antifungal susceptibility were correlated with patients’ HbA1c measurements. Results: The OCC and candidal density carriage was significantly higher in diabetics. Candida albicans (C. albicans) was the most frequently isolated species followed by Candida tropicalis (C. tropicalis). Relatively uncommon species, Candida lusitaniae (C. lusitaniae) and Candida lipolytica (C. lipolytica) were isolated from the diabetics. Prevalence of virulence factor, proteinase, was greater in diabetic group (p < 0.05). Reduced fluconazole susceptibility was noted among the isolates from diabetics; however it was not statistically significant (p = 0.593). Except one, all the susceptible-dose dependent and resistant isolates were Candida no-albicans (C. non-albicans). Conclusion: C. albicans remains the predominant pathogen in diabetics, although other species are on the rise. Compared to control group, the isolated species from T2DM group had higher proteinase activity. Resistance to fluconazole was considerably greater among the C. non-albicans isolates from T2DM group. These findings warrant effective treatment modalities to reduce the occurrence of oropharyngeal candidiasis.

Item Type: Article
Uncontrolled Keywords: Type 2 diabetes mellitus (T2DM); candida; virulence; fluconazole susceptibility; oral candidal carriage.
Subjects: Dentistry > MCODS Manipal > Oral Pathology and Microbiology
Medicine > KMC Manipal > Medicine
Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: KMC Library
Date Deposited: 24 Aug 2017 11:04
Last Modified: 24 Aug 2017 11:04
URI: http://eprints.manipal.edu/id/eprint/149580

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