Real time monitoring of biomolecular interactions using Lab-Built surface plasmon resonance (SPR) instrument

Lukose, Jijo and Kulal, V and Santhosh, Chidangil and Sinha, Rajeev K (2017) Real time monitoring of biomolecular interactions using Lab-Built surface plasmon resonance (SPR) instrument. Advanced Science Letters, 23 (3). pp. 1807-1810. ISSN 1936-6612

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Abstract

Surface Plasmon Resonance based sensors have found appreciable application in the biochemical and biological research due to its capability of real time monitoring of biomolecular interactions. In this study, we report the label free detection of low weight biological analytes in real time using a lab-built surface plasmon resonance based instrument. Real time binding of biotin to streptavidin attached on sensor chip was monitored in terms of variation in reflected light intensity. Mixed self-assembled monolayer of 1-Octanethiol and 1,10-Decanedithiol was explored as a linker layer for streptavidin attachment on metal film surface. The result shows that developed surface plasmon resonance based instrument has greater potential for detection of the low weight biological analytes as well as monitoring the biomolecular interactions in real time applications.In future this device can be used to investigate about the binding kinetics of bimolecular interactions to resolve the kinetic constants.

Item Type: Article
Uncontrolled Keywords: Biotin; self assembled monolayer; streptavidin; surface plasmon resonance.
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Library
Date Deposited: 31 Aug 2017 09:18
Last Modified: 31 Aug 2017 09:18
URI: http://eprints.manipal.edu/id/eprint/149613

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