Polarization resolved second harmonic microscopy

Mazumder, Nirmal (2017) Polarization resolved second harmonic microscopy. Methods, 128 (1). pp. 105-118. ISSN 1046-2023

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Abstract

Second harmonic (SH) microscopy has proven to be a powerfulimagingmodality over the past years due to its intrinsic advantages as a multiphoton process with endogenous contrast specificity, which allows pinholeless optical sectioning, non-invasive observation, deep tissue penetration, and the possibility of easier signal detection at visible wavelengths. Depending on the relative orientation between the polarization of the incoming light and the second-order susceptibility of non-centrosymmetric structures, SH microscopy provides the unique capacity to probe the absolute molecular structure of a broad variety of biological tissues without the necessity for additional labeling. In addition, SH microscopy, when working with polarimetry, provides clear and in-depth insights on the details of molecular orientation and structural symmetry. In this review, theworking principles of the polarization resolving techniques and the correspondingimplements of SH microscopy are elucidated, with focus on Stokes vector based polarimetry. An overview of the advancements on SH anisotropy measurements are also presented. Specifically, the recent progresses on the following three topics in polarization resolved SH microscopy will be elucidated, which include Stokes vector resolving for imaging molecular structure and orientation, 3-D structural chirality by SH circular dichroism, and correlation with fluorescence lifetime imaging (FLIM) for in vi o wound healing diagnosis. The potentials and challenges for future researches in exploring complex biological tissues are also discussed.

Item Type: Article
Uncontrolled Keywords: Optical scanning microscopy; second harmonic; stokes vector; polarization; chirality; fluorescence lifetime imaging (FLIM).
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 18 Sep 2017 03:47
Last Modified: 18 Sep 2017 03:47
URI: http://eprints.manipal.edu/id/eprint/149700

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