Assessment of free radical scavenging and anti-proliferative activities of tinospora cordifolia miers (Willd)

Rao, Picheswara P and Udupa, Nayanabhirama and Khan, Saleemulla and Maheswari, Rajlexmi (2017) Assessment of free radical scavenging and anti-proliferative activities of tinospora cordifolia miers (Willd). BMC Complementary and Alternative Medicine, 17 (457). pp. 1-12. ISSN 1472-6882

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Abstract

Background: Tinospora cordifolia (Guduchi or Amrita) is an important drug of Ayurvedic System of Medicine and found mention in various classical texts for the treatment of diseases such as jaundice, fever, diabetes, cancer and skin disease etc. In view of its traditional claims, antioxidant and anti-proliferative activities were evaluated in the present study. Methods: Ethanol extract (TCE) and subsequent petroleum ether (TCP), dichloromethane (TCD), n-Butanol (TCB) and aqueous (TCA) fractions of were prepared from stems of T cordifolia. Total phenolic, flavonoid content and antioxidant activity was assessed by different methods. Anti-proliferative activity was assessed in cervical carcinoma (HeLa) cell lines by MTT and SRB assay. Results: Ethanol extract and n-butanol fractions shown to be superior in their scavenging activity in all the tested methods. n-butanol fractions shown antioxidant activity with an IC50 of 14.81 ± 0.53, 29.48 ± 2.23, 58.20 ± 0.70 and 21.17 ± 1.19 μg/mL by DPPH, ABTS, Nitric oxide and iron chelating activities respectively. Anti-proliferative activity results demonstrates that the TCD and ethanol extract of T cordifolia exhibits potent cytotoxic effect against HeLa with an IC50 of 54.23 ± 0.94 μg/mL and 101.26 ± 1.42 μg/mL respectively by MTT assay; and with an IC50 of 48. 91 ± 0.33 μg/mL and 87.93 ± 0.85 μg/mL respectively by SRB assay. Conclusion: The outcomes of the present study support the fact that T Cordifolia is a promising source of antioxidant agent and propose its further investigation. Moreover, dichloromethane fraction of T cordifolia shown to be the most potent anti-proliferative fraction and further mechanistic and phytochemical investigations are under way to identify the active principles.

Item Type: Article
Uncontrolled Keywords: Tinospora cordifolia; Anti-oxidant; Berberine; Cervical carcinoma; SRB assay.
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Pharmacy > MCOPS Manipal > Pharmacy Management
Research > Research Center - Health Sciences
Depositing User: KMC Library
Date Deposited: 25 Sep 2017 04:34
Last Modified: 25 Sep 2017 04:34
URI: http://eprints.manipal.edu/id/eprint/149736

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