Development and validation of an hplc analytical method for the detection and quantification of nicardipine in lipid nanoparticles

Fernandes, Amanda V and Pydi, Chinna R and Pai, Girish K and Verma, Ruchi and Kumar, Lalit (2017) Development and validation of an hplc analytical method for the detection and quantification of nicardipine in lipid nanoparticles. Latin American Journal of Pharmacy, 36 (10). pp. 1995-2000. ISSN 0326- 2383

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Abstract

SUMMARY. An HPLC method was developed and validated as per ICH guidelines for nicardipine analysis in lipid nanoparticles. Nicardipine was quantified using methanol and 40 mM ammonium formate (pH 3.5) with a ratio 65:35% v/v and BDS C18 column as a stationary phase. Detection of drug was carried out using UV detector at 240 nm by maintaining the flow rate at 1.0 mL/min. Retention time of nicardipine was found to be 4.67 min. Linearity was constructed between 0.50 to 32.00 μg/mL. Percent relative standard deviation (%RSD) from intraday and interday precisions were found to be less than 1% and 2%, respectively.. Nicardipine recovery was found from 93.94 ± 0.51 to 100.16 ± 0.18%. LOD and LOQ values were found to be 25.50 ng/mL and 77.28 ng/mL, respectively. Robustness studies also confirmed that developed method is robust with %RSD < 2%. Study concludes that developed method can be used for analysis of nicardipine in lipid nanoparticles.

Item Type: Article
Uncontrolled Keywords: Analytical method; antihypertensive drug; calcium channel blocker; HPLC; nanoparticulate drug delivery system; nicardipine; NLCs; SLNs.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 06 Oct 2017 11:19
Last Modified: 06 Oct 2017 11:19
URI: http://eprints.manipal.edu/id/eprint/149761

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