Characterization of the phenolic compound, gallic acid from sansevieria roxburghiana schult and schult. f. rhizomes and antioxidant and cytotoxic activities evaluation

Maheshwari, Rajalekshmi and Shreedhara, CS and Rao, Picheswara P and Managuli, Renuka S and Xavier, Seena Kanniparambil and Richard, L and Setty, Manjunath M and Mutalik, Srinivas (2017) Characterization of the phenolic compound, gallic acid from sansevieria roxburghiana schult and schult. f. rhizomes and antioxidant and cytotoxic activities evaluation. Pharmacognosy Magazine, 13 (51-S3). S693-S699. ISSN 0973-1296

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Abstract

Background: Sansevieria roxburghiana Schult. and Schult. f. (Asparagaceae) grows in India, Indonesia, Sri Lanka, and tropical Africa. Even though the plant has been traditionally used for the treatment of many ailments, the antioxidant and antiproliferative activities of S. roxburghiana methanol extract and its fractions have not yet been explored. Materials and Methods: Quantitative estimation of phenols and different antioxidant assays were performed using standard methods. Anti‑proliferative effect of the extract and fractions were evaluated in HCT‑116, HeLa, MCF‑7, HepG2, and A‑549 cancer cell lines by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) and sulforhodamine B (SRB) assay methods. High‑performance liquid chromatography (HPLC) and high‑performance thin layer chromatography (HPTLC) fingerprint profiling were carried out for extract and different fractions. Results: Significant antioxidant and anti‑proliferate activity were detected in ethyl acetate fraction. Ethyl acetate fraction showed prominent scavenging activity in 1,1‑diphenyl‑2‑picrylhydrazyl, 2,2′‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulfonic acid) diammonium salt, and nitric oxide antioxidant assays with an concentration yielding 50% inhibition (IC50) 15.33 ± 1.45, 45.3 ± 1.93 and 48.43 ± 0.46 μg/ml, respectively. Cytotoxicity of ethyl acetate fraction was the highest among other fractions against HCT‑116, HeLa, and MCF‑7cancer cell lines with IC50 values 16.55 ± 1.28, 12.38 ± 1.36, and 8.03 ± 1.9 μg/ml, respectively, by MTT assay and 15.57 ± 0.70, 13.19 ± 0.49, and 10.34 ± 0.9 μg/ml, respectively, by SRB assay. The presence of gallic acid in the ethyl acetate fraction of S. roxburghiana rhizomes was confirmed by HPLC and HPTLC analysis. Conclusion: Results suggested that ethyl acetate fraction exhibited effective antioxidant and antiproliferative activities. The phenolic compounds identified in ethyl acetate fraction could be responsible for the activities.

Item Type: Article
Uncontrolled Keywords: Antioxidant; antiproliferative; high‑performance liquid chromatography; high‑performance thin layer chromatography; Sansevieria roxburghiana.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutics
Pharmacy > MCOPS Manipal > Pharmacognosy
Depositing User: KMC Library
Date Deposited: 13 Oct 2017 09:42
Last Modified: 13 Oct 2017 09:42
URI: http://eprints.manipal.edu/id/eprint/149811

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