Therapeutic potential of human bone marrow derived mesenchymal stromal cells in combination with silymarin against carbon tetrachloride induced liver cirrhosis in wistar rats

Aithal, Ashwini P and Kumar, Naveen (2017) Therapeutic potential of human bone marrow derived mesenchymal stromal cells in combination with silymarin against carbon tetrachloride induced liver cirrhosis in wistar rats. Journal of Krishna Institute of Medical Sciences University, 6 (4). pp. 38-49. ISSN 2231-4261

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Abstract

Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl ) induced liver 4 cirrhosis in Wistar rats. Material and Methods: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl . Sixty 4 rats were randomly divided into 6 groups: Group 1 (Normal Control Group), Group 2 (received only CCl ), Group 3 (CCl +low dose BM-MSCs), Group 4 4 4 (CCl +high dose BM-MSCs), Group 5 (CCl + 4 4 silymarin), Group 6 (CCl +BM-MSCs+silymarin). On 4 day 0 and at the end of 6th, 12th, 18th, 24th and 30th day after treatment, blood samples were collected for liver enzyme estimations. After 30 days of treatment, the rats were sacrificed; livers were excised and used for antioxidant analysis and histopathological studies. Results: Liver enzyme analysis and histopathological studies indicated that combination of BM-MSCs and silymarin was effective in treating liver cirrhosis. Furthermore, oxidative stress was attenuated in the group which received combination treatment of BMMSCs and silymarin. Conclusion: Evidence of this study showed that combination treatment of BM Therapeutic MSCs and silymarin was beneficial and support the potential of using MSCs transplantation as an effective treatment modality for liver cirrhosis.

Item Type: Article
Uncontrolled Keywords: Liver Cirrhosis; Mesenchymal Stromal Cells; Transplantation; Carbon tetrachloride; Silymarin.
Subjects: Medicine > MMMC Manipal > Anatomy
Depositing User: KMC Library
Date Deposited: 21 Oct 2017 03:42
Last Modified: 21 Oct 2017 03:42
URI: http://eprints.manipal.edu/id/eprint/149841

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