PLGA nanofibers blended with designer self-assembling peptides for peripheral neural regeneration

Nune, Manasa (2016) PLGA nanofibers blended with designer self-assembling peptides for peripheral neural regeneration. Materials Science and Engineering C, 62. pp. 329-337. ISSN 0928-4931

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Abstract

Electrospun nanofibers are attractive candidates for neural regeneration due to similarity to the extracellular matrix. Several synthetic polymers have been used but they lack in providing the essential biorecognition motifs on their surfaces. Self-assembling peptide nanofiber scaffolds (SAPNFs) like RADA16 and recently, designer SAPs with functional motifs RADA16-I-BMHP1 areexamples, which showed successful spinal cord regeneration. But these peptide nanofiber scaffolds have poor mechanical properties and faster degradation rates that limit their use for larger nerve defects. Hence, we have developed a novel hybrid nanofiber scaffold of polymer poly(Llactide- co-glycolide) (PLGA) and RADA16-I-BMHP1. The scaffolds were characterized for the presence of peptides both qualitatively and quantitatively using several techniques like SEM, EDX, FTIR, CHN analysis, Circular Dichroism analysis, Confocal and thermal analysis. Peptide self-assembly was retained post-electrospinning and formed rod-like nanostructures on PLGA nanofibers. In vitro cell compatibilitywas studied using rat Schwann cells and their adhesion, proliferation and gene expression levels on the designed scaffolds were evaluated. Our results have revealed the significant effects of the peptide blended scaffolds on promoting Schwann cell adhesion, extension and phenotypic expression. Neural development markers (SEM3F, NRP2 & PLX1) gene expression levels were significantly upregulated in peptide blended scaffolds compared to the PLGA scaffolds. Thus the hybrid blended novel designer scaffolds seemto be promising candidates for successful and functional regeneration of the peripheral nerve.

Item Type: Article
Uncontrolled Keywords: Peripheral nerve injury ; Electrospun nanofibers ; Self-assembling peptides ; Poly(L-lactide-co-glycolide) (PLGA) ; RADA16-I-BMHP1 peptide.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 10 Nov 2017 09:10
Last Modified: 10 Nov 2017 09:10
URI: http://eprints.manipal.edu/id/eprint/149940

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