Molecular mechanisms underlying gliomas and glioblastoma pathogenesis revealed by bioinformatics analysis of microarray data

Chandrashekar, Raghu (2017) Molecular mechanisms underlying gliomas and glioblastoma pathogenesis revealed by bioinformatics analysis of microarray data. Medical Oncology, 34 (11). pp. 1-30. ISSN 1357-0560

[img] PDF
00003464.pdf - Published Version
Restricted to Registered users only

Download (4MB) | Request a copy

Abstract

The aim of this study was to identify key genes associated with gliomas and glioblastoma and to explore the related signaling pathways. Gene expression profiles of three glioma stem cell line samples, three normal astrocyte samples, three astrocyte overexpressing 4 iPSC-inducing and oncogenic factors (myc(T58A), OCT-4, p53DD, and H-Ras(G12V)) samples, three astrocyte overexpressing 7 iPSC-inducing and oncogenic factors (OCT4, H-Ras(G12V), myc(T58A), p53DD, cyclin D1, CDK4(RC24) and hTERT) samples and three glioblastoma cell line samples were downloaded from the ArrayExpress database (accession: E-MTAB-4771). The differentially expressed genes (DEGs) in gliomas and glioblastoma were identified using FDR and t tests, and protein–protein interaction (PPI) networks for these DEGs were constructed using the protein interaction network analysis. The GeneTrail2 1.5 tool was used to identify potentially enriched biological processes among the DEGs using gene ontology (GO) terms and to identify the related pathways using the Kyoto Encyclopedia of Genes and Genomes, Reactome and WikiPathways pathway database. In addition, crucial modules of the constructed PPI networks were HOXA10 and MET, may be used for targeted diagnosis and treatment of gliomas and glioblastoma.

Item Type: Article
Uncontrolled Keywords: DEGs ; Gliomas ; Glioblastoma ; Protein– protein interaction network ; Transcription factors ; MicroRNA.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: KMC Library
Date Deposited: 22 Nov 2017 10:31
Last Modified: 22 Nov 2017 10:31
URI: http://eprints.manipal.edu/id/eprint/150039

Actions (login required)

View Item View Item