Sperm-derived Factors Enhance the in Vitro Developmental Potential of Haploid Parthenotes

Nair, Ramya and Aboobacker, Shahin and Mutalik, Srinivas and Kalthur, Guruprasad and Adiga, Satish Kumar (2017) Sperm-derived Factors Enhance the in Vitro Developmental Potential of Haploid Parthenotes. Zygote, 28. pp. 1-14. ISSN 0967-1994

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Parthenotes are characterized by poor in vitro developmental potential either due to the ploidy status or the absence of paternal factors. In the present study, we demonstrate the beneficial role of spermderived factors (SDF) on the in vitro development of mouse parthenotes. Mature (MII) oocytes collected from superovulated Swiss albino mice were activated using strontium chloride (SrCl2) in the presence or absence of various concentrations of SDF in M16 medium. The presence of SDF in activation medium did not have any significant influence on the activation rate. However, a significant increase in the developmental potential of the embryos and increased blastocyst rate (P < 0.01) was observed at 50 μg/ml concentration.Furthermore, the activated oocytes from this group exhibited early cleavage and cortical distribution of cortical granules that was similar to that of normally fertilized zygotes. Culturing 2-cell stage parthenotes in the presence of SDF significantly improved the developmental potential (P < 0.05) indicating that they also play a significant role in embryo development. In conclusion, artificial activation of oocytes with SDF can improve the developmental potential of parthenotes in vitro

Item Type: Article
Uncontrolled Keywords: Cortical reaction; Developmental potential; Mitochondria; Parthenotes; Sperm-derived factor; Spindle assembly
Subjects: Medicine > KMC Manipal > Clinical Embryology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 04 Dec 2017 04:28
Last Modified: 17 Aug 2021 09:21
URI: http://eprints.manipal.edu/id/eprint/150111

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