Phase modulated 2D HSQC-TOCSY for unambiguous assignment of overlapping spin systems

Adiga, Satish Kumar (2017) Phase modulated 2D HSQC-TOCSY for unambiguous assignment of overlapping spin systems. Journal of Magnetic Resonance, 286 (2). pp. 10-16. ISSN 1090-7807

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We present a new method that allows one to unambiguously resolve overlapping spin systems often encountered in biomolecular systems such as peptides and proteins or in samples containing a mixture of different molecules such as in metabolomics. We address this problem using the recently proposed phase modulation approach. By evolving the 1H chemical shifts in a conventional two dimensional (2D) HSQC-TOCSY experiment for a fixed delay period, the phase/intensity of set of cross peaks belonging to one spin system are modulated differentially relative to those of its overlapping counterpart, resulting in their discrimination and recognition. The method thus accelerates the process of identification and resonance assignment of individual compounds in complex mixtures. This approach facilitated the assignment of molecules in the embryo culture medium used in human assisted reproductive technology.

Item Type: Article
Uncontrolled Keywords: HSQC-TOCSY; phase modulation; high resolution NMR; metabolomics; fast NMR methods.
Subjects: Medicine > KMC Manipal > Clinical Embryology
Depositing User: KMC Library
Date Deposited: 04 Dec 2017 06:11
Last Modified: 17 Aug 2021 09:19

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