Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activity

Rajeshwari, HR (2018) Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activity. ACS Biomaterials-Science & Engineering, 4 (3). pp. 892-899. ISSN 2373-9878

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Abstract

Silver nanoparticles (SNPs), owing to their wide range of biomedical applications, have recently attracted remarkable interest for use in cancer nanomedicine. The present research work investigated the anticancer activity of phytosynthesized SNPs against human cancer cell lines. Phytosynthesis of SNPs was achieved by using an aqueous extract of Salacia chinensis (SC) bark as a green source to reduce silver nitrate to silver nanoparticles. Characterization of synthesized nanoparticles demonstrated a UV−visible peak at 443 nm, ζ-potential (zetasizer) of −25.6 ± 0.34 and particle size (transmission electron microscopy analysis) in the range of 40−80 nm, which validates formation of stable silver nanoparticles. The absence of cytotoxicity against normal human fibroblasts and blood erythrocytes confirms the biocompatible nature of green synthesized SNPs. In vitro anticancer assay demonstrated IC50 values of 6.31, 4.002, 5.228, 8.452, 14.37, 7.46, and 6.55 μg/ mL against liver (Hep G2), lungs (L-132), pancreas (MIA-Pa-Ca-2), breast (MDA-MB-231), oral (KB cells), prostate (PC-3), and cervical (HeLa) cancer cell lines respectively, which confirms its potent anticancer action. The results of the present study give an experimental proof that the SC mediated green synthesized SNPs could serve as a promising anticancer agent to overcome limitations of existing conventional cancer chemotherapeutics.

Item Type: Article
Uncontrolled Keywords: Silver nanoparticles; green chemistry; anticancer activity; biocompatibility; Salacia chinensis.
Subjects: Dentistry > MCODS Manipal > Periodontology
Depositing User: KMC Library
Date Deposited: 21 May 2018 05:58
Last Modified: 21 May 2018 05:58
URI: http://eprints.manipal.edu/id/eprint/151020

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