Real-time and rapid detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance setup

Lukose, Jijo and Shetty, Vignesh and Ballal, Mamatha and Chidangil, Santhosh and Sinha, Rajeev K (2018) Real-time and rapid detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance setup. Laser Physics Letters, 15. pp. 1-7. ISSN 1612-2011

[img] PDF
4506 DisplayPdf.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Cost-effective diagnostic platforms for rapid pathogen detection are always incumbent in both developing and developed worlds. However, exorbitant diagnostic expenses and the inability to detect pathogens early are a matter of concern for the sustainability and affordability of healthcare devices, which are crucial for deciding how to provide healthcare solutions to the masses, especially in developing countries. Herein, we present the rapid and realtime detection of Salmonella Typhimurium using an inexpensive lab-built surface plasmon resonance (SPR) imaging set up. Pathogen detection is accomplished with the aid of a monoclonal antibody immobilized on a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide): N-hydroxysuccinimide-modified self-assembled monolayer covalently bonded to a Au thin film. Successful pathogen detection is performed at two concentrations, ~1.5 × 108 and ~1 × 106 cfu ml−1, in phosphate-buffered saline solution. The developed system is capable of detecting bacterial cells within 6–7 min after their injection into the SPR sensor surface. The present study reveals a cost-effective device having high potential for pathogen detection without any labelling tags.

Item Type: Article
Uncontrolled Keywords: Surface plasmon resonance imaging; Salmonella Typhimurium; Self-assembled monolayer; Alkanethiol
Subjects: Departments at MU > Atomic Molecular Physics
Medicine > KMC Manipal > Microbiology
Depositing User: KMC Library
Date Deposited: 06 Jun 2018 06:30
Last Modified: 06 Jun 2018 06:30
URI: http://eprints.manipal.edu/id/eprint/151248

Actions (login required)

View Item View Item