Evaluation of p53 protein expression in Barrett esophagus

Krothapalli, Mahathi and Kini, Jyoti R. and Kini, Hema and *, Kausalya Kumari Sahu and Shenoy, Suresh and *, Sandeep Gopal Krishna and Tantry, Vishwanath B (2018) Evaluation of p53 protein expression in Barrett esophagus. Indian Journal of Pathology and Microbiology, 6 1 (2). pp. 170-175. ISSN 03774929

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Abstract

Background: Loss of heterozygosity of p53 along with aneuploidy is deemed to be the early molecular steps in Barrett metaplasia‑dysplasia‑adenocarcinoma sequence. Objective biomarkers need to be used along with microscopy for risk stratification to predict the progression of Barrett esophagus (BE) to carcinoma. Aim: This study aims to study p53 protein expression in dysplasia and correlate the same with morphology in BE. Materials and Methods: A time‑bound study was conducted from January 2011 to June 2015. All esophageal biopsies showing histological evidence of columnar epithelium with the presence of goblet cells were included. The cases which showed dysplasia were graded on hematoxylin and eosin stain. Evaluation of p53 immunohistochemistry staining was done on all the cases of BE. Dysplasia was correlated with the expression of p53 using Chi‑square value (χ2) and Fischer’s exact test wherever appropriate. P < 0.05 was considered to be statistically significant. Results: Of 829 esophageal biopsies received, 119 were endoscopically suspected to be BE, of which 85 cases were confirmed on microscopy. In our study, there were 75 cases negative for dysplasia (88.2%), 8 with low‑grade dysplasia (LGD) (9.4%), and two with high‑grade dysplasia (HGD) (2.4%). Three cases of BE had associated adenocarcinoma. Immunostaining with p53 done on all the 85 cases showed positive staining in all cases with LGD, one with HGD and two with adenocarcinoma. In the present study, immunostaining with p53 showed 90% sensitivity, 89.3% specificity, positive predictive value of 52.9%, and negative predictive value of 98.5%. Conclusion: The technical simplicity, easy availability, and comparatively lower cost enhance the role of p53 as a biomarker in risk stratification for patients with BE.

Item Type: Article
Uncontrolled Keywords: Barrett esophagus, dysplasia, esophageal adenocarcinoma, p53
Subjects: Medicine > KMC Mangalore > Gastroenterology
Medicine > KMC Mangalore > Pathology
Depositing User: KMCMLR User
Date Deposited: 03 Jul 2018 05:53
Last Modified: 03 Jul 2018 05:53
URI: http://eprints.manipal.edu/id/eprint/151465

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