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A Pilot Study on Colonic Mucosal Tissues by Fluorescence Spectroscopy Technique: Discrimination by Principal Component Analysis (PCA) And Artificial Neural Network (ANN) Analysis

Kamath, Sudha D and D’souza, Claretta S and Mathew, Stanley and George, Sajan D and Santhosh, C and Mahato, KK (2008) A Pilot Study on Colonic Mucosal Tissues by Fluorescence Spectroscopy Technique: Discrimination by Principal Component Analysis (PCA) And Artificial Neural Network (ANN) Analysis. J. Chemometrics, 22. pp. 408-416.

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Abstract

Pulsed laser-induced autofluorescence spectra of pathologically certified normal and malignant colonic mucosal tissues were recorded at 325nm excitation. The spectra were analysed using three different methods for discrimination purposes. First, all the spectra were subjected to the principal component analysis (PCA) and the discrimination between normal and malignant cases were achieved using parameters like, spectral residuals, Mahalanobis distance and scores of factors. Second, to understand the changes in tissue composition between the two classes (normal, and malignant), difference spectrum was constructed by subtracting mean spectrum of calibration set samples from simulated mean of all spectra of any one class (normal/malignant) and in third, artificial neural network (ANN) analysis was carried out on the same set of spectral data by training the network with spectral features like, mean, median, spectral residual, energy, standard deviation, number of peaks for different thresholds (100, 250 and 500) after carrying out 1st-order differentiation of the training set samples and discrimination between normal and malignant conditions were achieved. The specificity and sensitivity were determined in PCA and ANN analyses and they were found to be 100 and 91.3% in PCA, and 100 and 93.47% in ANN, respectively.

Item Type: Article
Uncontrolled Keywords: Colonic mucosa, Laser-induced fluorescence, Principal component analysis, Artificial neural network
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Surgery
Depositing User: KMC Manipal
Date Deposited: 15 Oct 2011 04:20
Last Modified: 15 Oct 2011 04:20
URI: http://eprints.manipal.edu/id/eprint/1518

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