Parameter optimization of a laser-induced fluorescence system for in vivo screening of oral cancer

Unnikrishnan, VK and Nayak, Rajesh and Bernard, Rodney and Priya, Jeena K and Patil, Ajeetkumar and George, Sajan D and Kartha, VB and Santhosh, C and Ebenezer, J and Pai, Keerthilatha M (2011) Parameter optimization of a laser-induced fluorescence system for in vivo screening of oral cancer. Journal of Laser Applications, 23 (3).

[img] PDF
Laser_application.pdf
Restricted to Registered users only

Download (722kB) | Request a copy

Abstract

Cancer is a disease that displays abnormal growth in cells, intrusion, and destruction of the adjacent tissues, and often metastasis to other locations in the body via lymph or blood. These three properties differentiate cancer from benign tumors. According to American Cancer Society, cancer accounts for 13% of overall deaths in the world. Early detection can reduce the mortality rate of cancer to a considerable extent. Efforts for developing an ideal tool for early in vivo cancer detection using the optical techniques have been going on in several laboratories across the world for last several years. Among various methods available, laserinduced fluorescence {LIF} is found to be more popular due to a better understanding of the technique as well as maturity of the state of art research. Although exogenous and endogenous fluorophores can be employed for the early detection, autofluorescence based techniques are more acceptable for in vivo investigations, since chemicals used for exogenous fluorescence can have deleterious side effects lasting over long periods. Autofluorescence technique relies mainly on variation in composition of fluorophores present in the tissue, during progression of disease.It has been reported that with the usage of appropriate algorithms, this technique can give very good discrimination between normal and cancerous tissues with great sensitivity and specificity. However, spectral features depend not only on the biofluorophores and their modification during carcinogenesis but also on their interactions with the microenvironment of regions of observation. They are also influenced by the design and characteristics of components of the experimental setup as well as the methods of observation. As a result, sensitivity, specificity, and accuracy of the detection technique based on the spectral features can be affected by external variables affecting the experimental system. Hence it is necessary to carry out a systematic investigation of the influence of the experimental setup and the parameters involved, on the spectral features, before finalizing the design of a device for in vivo detection of cancer.

Item Type: Article
Uncontrolled Keywords: Oral cancer, laser-induced fluorescence, fiber probe
Subjects: Dentistry > MCODS Manipal > Oral Medicine and Radiology
Departments at MU > Atomic Molecular Physics
Depositing User: KMC Manipal
Date Deposited: 15 Oct 2011 04:10
Last Modified: 15 Oct 2011 04:10
URI: http://eprints.manipal.edu/id/eprint/1519

Actions (login required)

View Item View Item