Analytical method development and validation for hplc-ecd determination of moxifloxacin in marketed formulations

Reddy, Phani Sekhar G and Navyasree, KS and Jagadish, PC and Bhat, Krishnamurthy (2018) Analytical method development and validation for hplc-ecd determination of moxifloxacin in marketed formulations. Pharmaceutical Chemistry Journal, 52 (7). pp. 674-679. ISSN 0091-150X

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Abstract

An HPLC-ECD analytical method with high reproducibility and wide linearity range has been developed and validated. Moxifloxacin was separated and identified using this method with a simple mobile phase comprising Britton Robinson buffer pH 5.0 and methanol (93 : 7 v/v) flowing at a rate of 0.5 mL/min through Acclaim C 18 column (150 mm � 4.6 mm � 5 ìm) maintained at 35°C and detected at redox potential value of 1.0 V. The LOD and LOQ were found to be 2.2 and 6.6 �g/mL, respectively. The HPLC-ECD method of moxifloxacin analysis was validated as per ICH Q 2 R 1 guidelines and applied for assay of marketed moxifloxacin formulations to establish the acceptable recovery.

Item Type: Article
Uncontrolled Keywords: Moxifloxacin; electrochemical detector; voltammetry, HPLC-ECD; redox potential.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Library
Date Deposited: 13 Nov 2018 04:34
Last Modified: 13 Nov 2018 04:34
URI: http://eprints.manipal.edu/id/eprint/152258

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