Whole-genome sequencing reveals genetic signature of bedaquiline resistance in a clinical isolate of Mycobacterium tuberculosis

Chawla, Kiran and Kumar, Ajay and Shenoy, Vishnu Prasad (2018) Whole-genome sequencing reveals genetic signature of bedaquiline resistance in a clinical isolate of Mycobacterium tuberculosis. Journal of Global Antimicrobial Resistance, 15. pp. 103-104. ISSN 22137165

[img] PDF
5233 DisplayPdf.pdf - Published Version
Restricted to Registered users only

Download (221kB) | Request a copy

Abstract

Objectives: The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis strains has opened up new challenges for tuberculosis (TB) control in India.This study examined molecular markers of resistance to bedaquiline, a new antituberculous drug with the potential to dramatically improve MDR-TB treatment outcomes and to reduce mortality.Methods: A clinical M. tuberculosis isolate with a MDR-TB profile was subjected to whole-genome sequencing using an Illumina NextSeq500 platform, followed by genome-wide sequence analysis.Results: A mutation in the Rv0678 coding region associated with in vitro bedaquiline resistance was identified. The strain represented the Delhi/CAS lineage.Conclusions: This first report of a potentially bedaquiline-resistant M. tuberculosis strain in India highlights the role of genome-wide sequence analysis of isolates from TB cases with a history of treatment in countries with a high burden of MDR-TB and XDR-TB.

Item Type: Article
Uncontrolled Keywords: Mycobacterium tuberculosis; Drug resistance; Bedaquiline; High-burden country; Tuberculosis; Genome sequencing
Subjects: Medicine > KMC Manipal > Microbiology
Depositing User: KMC Library
Date Deposited: 13 Nov 2018 09:53
Last Modified: 13 Nov 2018 09:53
URI: http://eprints.manipal.edu/id/eprint/152269

Actions (login required)

View Item View Item