A laser Raman tweezers study of eryptosis

Barkur, Surekha and Mathur, Deepak and Santhosh, C (2018) A laser Raman tweezers study of eryptosis. Journal of Raman Spectroscopy, 49 (7). pp. 1-10. ISSN 0377-0486

[img] PDF
5375 DisplayPdf.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Eryptosis—the suicidal death of erythrocytes—is characterized by membrane blebbing and cell shrinkage. Eryptosis can be triggered by various xenobiotics such as carbon monoxide, lead, and amyloid, and by stressors such as oxidative stress, osmotic shock, and rapid alteration of ambient conditions. We have used Raman tweezers spectroscopy to study eryptosis in single, live cells and have attempted to explore the underlying mechanism, specifically to identify possible Raman signatures of eryptosis. Erythrocytes (red blood cells) were exposed to free radicals, silver nanoparticles, glucose, heat, and osmotic shock to induce eryptosis, and a comparison was made of their Raman spectra, which indicated that these conditions lead to a transition of haemoglobin from the R to the T state. Consequences of eryptosis include dehydration, cell shrinkage, and pH changes, which result in deoxygenation of haemoglobin. This, in turn, can be detected by monitoring the wavenumber shifts associated with Raman marker bands of R to T transitions. In addition, the principal component analysis results indicate differentiation among red blood cells undergone eryptosis due to different conditions.

Item Type: Article
Uncontrolled Keywords: Eryptosis; Erythrocytes; Haemoglobin; Oxidative stress; Raman tweezers
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Library
Date Deposited: 22 Nov 2018 09:48
Last Modified: 22 Nov 2018 09:48
URI: http://eprints.manipal.edu/id/eprint/152332

Actions (login required)

View Item View Item