Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts

D’souza, Fiona and Uppangala, Shubhashree and Salian, Sujith Raj and Kalthur, Guruprasad and Adiga, Satish Kumar (2018) Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts. Scientific Reports, 8. pp. 1-10. ISSN 2045-2322

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Abstract

The metabolomic profle of an embryo culture medium can aid in the advanced prediction of embryonic developmental potential and genetic integrity. But it is not known if this technology can be used to determine the in vitro potential of inner cell mass (ICM) in adherence and proliferation. Here, we investigated the developmental potential of mouse 2-cell embryos carrying cisplatin-induced DNA lesions (IDL), beyond blastocyst stage using ICM outgrowth assay. The genetic integrity of ICM cells was determined by comet assay. The metabolic signatures of spent medium were recorded 84hours post injection of hCG (hpi-hCG), and after 96hours of extended in vitro culture (Ex 96) by NMR spectroscopy. We observed that blastocysts that lack the ability to adhere in vitro had an increased requirement of pyruvate (p<0.01), lactate (p<0.01), and were accompanied by a signifcant reduction of pyruvatealanine ratio in the culture medium. We propose that the aforementioned metabolites from 84 hpi-hCG spent medium be further explored using appropriate experimental models, to prove their potential as biomarkers in the prediction of implantation ability of in vitro derived human embryos in clinical settings.

Item Type: Article
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Library
Date Deposited: 10 Dec 2018 03:56
Last Modified: 10 Dec 2018 03:56
URI: http://eprints.manipal.edu/id/eprint/152441

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