Protein profile study of Pap smear and tissue of cervix by High Performance Liquid Chromatography- Laser Induced Fluorescence

Sujatha, * and Rai, Lavanya and Kumar, Pratap and Krishnanand, BR and Mahato, KK and George, Sajan D and Kartha, VB and Santhosh, C (2007) Protein profile study of Pap smear and tissue of cervix by High Performance Liquid Chromatography- Laser Induced Fluorescence. Proc. of SPIE, 6441. pp. 1-8.

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Abstract

HPLC combined with laser induced fluorescence provides a very sensitive method for the separation and identification of the many proteins present in clinical samples. Protein profiles of clinical samples like Pap smear and tissue samples, from subjects with cervical cancer and normal volunteers, were recorded using HPLC-LIF. The protein profiles were analyzed by Principal Component Analysis (PCA). The profiles were characterized by parameters like scores of the factors, sum of squared residuals, and Mahalanobis Distance, derived from PCA. Parameters of each sample were compared with those of a standard set and Match/ No Match results were generated. Good discrimination between normal and malignant samples was achieved with high sensitivity and specificity.

Item Type: Article
Uncontrolled Keywords: HPLC-LIF, Pap smear, Biopsy tissue, Cancer of the cervix and PCA
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Medicine > KMC Manipal > Pathology
Depositing User: KMC Manipal
Date Deposited: 12 Oct 2011 10:58
Last Modified: 12 Oct 2011 10:58
URI: http://eprints.manipal.edu/id/eprint/1528

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