Non-invasive assessment of fecal progestagens and pregnancy detection in Himalayan musk deer (Moschus chrysogaster)

Mithileshwari, Chandrashekar and Srivastava, Tanushree and Kumar, Vinod and Kumar, Ajith and Umapathy, Govindswamy (2016) Non-invasive assessment of fecal progestagens and pregnancy detection in Himalayan musk deer (Moschus chrysogaster). Theriogenolgy, 85. pp. 216-223.

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Abstract

The Himalayan musk deer (Moschus chrysogaster), an endangered species. is facing threat of extinction globally due to severe hunting for its musk. and efforts are under way in India; to breed them in captivity. However. no information is available on the reproductive cycles' of the species. In this study. we aimed to standardize an enzyme immunoassay (EIA) procedure for monitoring pregnancy using fecal samples. We collected fecal samples for 12 months from five captive females maintained at the Musk Deer Research Centre. Bageshwar. Uttarakhand, India. Three of these females were observed mating and gave birth. whereas two were seen mating but did not give birth. The gestation periods for the three females were 183. 185. and 199 days, respectively. High-pressure liquid chromatography revealed the presence of immunoreactive pregnanediol-3-glucuronide (PdG). progesterone. and 5a-pregnan-3a-ol-20-one (5-alpha-pregnane) metabolites in the fecal samples. We used EIAs against progesterone. PdG. and 5-alpha-pregnane to monitor pregnancy. We found PdG EIAto be a highly accurate and sensitive assay compared with the other two assays in detecting pregnancy. We conclude that PdG EIAcan be used to diagnose and monitor pregnancy in Himalayan musk deer using fecal steroid analysis. at an early stage of 3 months after mating. This study would help in conservation breeding of musk deer in captivity and in monitoring the reproductive status of the species in the wild.

Item Type: Article
Uncontrolled Keywords: Himalayan musk deer Fecal hormone Pregnancy Pregnanediol-3-glucuronide Progesterone 5o:-pregnan-3o:-ol-20-one
Subjects: Research > Research Center - Technical
Depositing User: MIT Library
Date Deposited: 05 Mar 2019 05:12
Last Modified: 05 Mar 2019 05:12
URI: http://eprints.manipal.edu/id/eprint/153392

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